ethyl violet的意思|示意
乙基紫
ethyl violet的用法详解
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Ethyl violet (also known as crystal violet, hexamethyl pararosaniline and gentian violet) is an organic compound used as a dye in medical diagnostic tests. It is also used in histology as a nuclear stain, as it stains the nucleus of cells a bright blue-violet color.
Ethyl violet is used to differentiate between gram-negative and gram-positive bacteria in a Gram stain test. Ethyl violet is added to a solution containing a bacterial suspension which is then applied to a microscope slide. The slide is then air dried and the bacteria are heat-fixed to the slide. The bacteria are then treated with a Gram stain reagent which will crystalize or colorize the bacteria. In the Gram stain test, gram-positive bacteria is stained a purple-violet color with ethyl violet and gram-negative bacteria is stained a red color with safranin.
In addition to its use in Gram staining, ethyl violet has a number of other uses. It is used in histology to stain nuclear material and as a fluorescent counterstain; it is an enzyme substrate and it can also be used in the identification of the alpha- and beta-hemolytic streptococci. It has also been used as a fungal stain and as an anti-fungal agent.
Ethyl violet has also been used in membrane-based assays. It is used to stain membranes in order to visualize the protein or lipid components of the membrane as well as to study the structure and composition of the membrane. It is also used to detect proteins, glycoproteins and lipids in membranes.
Overall, ethyl violet is an important dye that has multiple uses in medical diagnostics, histology and membrane-based assays. It is used to differentiate between gram-positive and gram-negative bacteria as well as to stain nuclear material and other components of cells. In addition, it has been used to identify the alpha- and beta-hemolytic streptococci, as an enzyme substrate and as an anti-fungal agent. Its use in membrane-based assays has allowed for research into the structure and composition of biological membranes.
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